PDF

Screening of Antitumor Bioactivity of Fungi Associated with Macro Algae and Sponge from Indrayanti Beach, Jogjakarta

---

This research was aimed to isolate marine derived-fungi which associated with macro algae and sponge from Indrayanti Beach, Jogjakarta and to screen the antitumor (T47D and HeLa) bioactivity of the fungi extracts. Three solid media of MEA (malt extract agar), GPY (glucose peptone yeast) and MFM (minimal fungi medium) were used as isolation medium. Each of the pure fungi isolates was then cultivated in 100 ml of liquid medium for 4 weeks at room temperature (27- 28°C) in static conditions. The antitumor activity of the fungi extracts were tested against breast tumor cells (T47D) and cervical cancer cells (HeLa) using Thiazolyl Blue Tetrazolium Blue (MTT) assay method. A number of 21 isolates of fungi were isolated from 4 macro algae and 1 sponge samples. The identification of fungi isolate was conducted using combination of molecular approach (ITS1-5.8S-ITS4 DNA regions) and macro-micro morphological characteristics. Among those 21 marine fungi species isolated, MFGK-21 extract showed the best anti-servical tumor (HeLa) with an IC 50 value of 240.1 μg/ml and MFGK-27 extract showed the best anti-breast tumor (T47D) with an IC 50 value of 59.6 μg/ml. The MFGK-21 fungi isolate was identified as Penicillium steckii, while the MFGK-27 fungi isolate was identified as Aspergillus sydowii.

---

Availability

B1801754

Koleksi Digital

Archivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan Perikanan

Available

Detail Information

Series Title

-

Call Number

Koleksi Digital

Publisher

2013 : KKP-Badan Penelitian dan Pengembangan Kelautan dan Perikanan., Jakarta

Collation

47-56 hlm.

Language

English

ISBN/ISSN

2089-5690

Classification

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edition

8 (2), 2013

Subject(s)

Local Content
Publikasi Internal
Produksi Bahan Aktif Anti Tumor dari Kapang Laut
Kapang
Makroalga
Spons

Specific Detail Info

-

Statement of Responsibility

Squalen Bulletin of Marine & Fisheries Postharvest & Biotechnology

Other version/related

File Attachment

Comments

---

You must be logged in to post a comment