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IDENTIFICATION AND PARTIAL CHARACTERIZATION OF CRUDE EXTRACELLULAR ENZYMES FROM BACTERIA ISOLATED FROM SHRIMP WASTE PROCESSING

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Attention on chitin degrading enzymes has been growing since their ability to reduce the waste of shrimp/other crustaceans processing industries and converting them into value added products such as biologically active chitin and chitosan oligomer. Previous experiment found that KLU 11.16 isolate was one of the potential bacteria isolated from shrimp waste producing chitinolytic enzymes including chitosanases. A study on the identification of KLU 11.16 extracellular crude enzyme was carried out by cultivating the bacteria on chitin medium. Due to the wide application of chitosanase, the characterization of the crude chitosanase was carried out after an identification of the enzymes secreted. Based on assessment using zymogram technique, this bacteria secreted a mixed extracellular chitinolytic enzyme and other hydrolytic enzyme. The crude chitinolytic enzyme degraded 85% deacetylated (DA) better than 100% DA chitosan, and slightly degraded glycol chitin, indicating that KLU 11.16 secreted chitosanases and chitinases enzyme. In addition to the chitinolytic enzyme, the bacteria also secreted protein and carbohydrate degrading enzymes when running at SDS-PAGE enriched with casein, soluble starch and CMC substrates. Crude chitosanases enzyme was performed well at pH 6 and temperature of 30 0 C, and the activity can be increased by addition of 1 mM Fe 2+ in form of chloride salt. Addition of detergent, i.e 1mM of Triton X-100 and SDS slightly decreased the activity. Future application of the crude chitosanase from KLU 11.16 was on producing chitosan derivative such as chitosan oligomer using substrate of 85% DA chitosan, which is more digestable by other enzymes secreted by KLU 11.16

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Availability

B1801661

Koleksi Digital

Archivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan Perikanan

Available

Detail Information

Series Title

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Call Number

Koleksi Digital

Publisher

2012 : KKP-Badan Penelitian dan Pengembangan Kelautan dan Perikanan., Jakarta

Collation

11-18 hlm.

Language

Indonesia

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

-

Carrier Type

-

Edition

Vol. 7 No. 1, May 2012

Subject(s)

Local Content
Publikasi Internal
Limbah Udang
Aktivitas Enzim Protase
Karya Ilmiah
Artikel Ilmiah
Bakteri -- Pemanfaatan

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Statement of Responsibility

Squalen Journal

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