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DISTRIBUTION AND EXPRESSION OF STRIPED CATFISH (Pangasionodon hypophtalmus) GROWTH HORMONE GENE (PhGH) IN THE ORGAN OF AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC FOUNDER (Indonesian Aquaculture Journal Vol.7 No.1, 2012)

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Faster growing African catfish can be produced by transgenesis. This study was conducted to investigate the distribution and expression of growth hormone gene (PhGH) in various organs of the transgenic African catfish (Clarias gariepinus) founder (F 0 ). Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, caudal fin, gill and eye. Transgene expression levels were analyzed using the method of reverse transcriptase-polymerase chain reaction (RT-PCR), -actin gene used as internal controls. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish founder. The high level of PhGH expression was found in the liver, pituitary, intestine and brain; smaller amounts were detectable in muscle, spleen, kidneys, heart, and stomach, caudal fin, gill and eyes, range from 0.02-0.75 PhGH/-actin mRNA. The expression levels of PhGH had positive correlation with tissue and body size (P < 0.05).

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Ketersediaan

B1801316

Koleksi Digital

Archivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan Perikanan

Tersedia

Detail Information

Judul Siri

-

No. Panggilan

Koleksi Digital

Penerbit

2012 : KKP-Badan Penelitian dan Pengembangan Kelautan dan Perikanan., Jakarta

Penerangan Fizikal

11-18 hlm.

Bahasa

Indonesia

ISBN/ISSN

-

Klasifikasi

NONE

Content Type

-

Media Type

-

Carrier Type

-

Edisi

Vol.7 No.1, 2012

Subjek

Jurnal
Local Content
Publikasi Internal
Clarias gariepinus
Transgenik

Maklumat khusus

-

Penyataan Tanggungjawab

Indonesian Aquaculture Journal

Other version/related

Fail Lampiran

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