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DISTRIBUTION AND EXPRESSION OF STRIPED CATFISH (Pangasionodon hypophtalmus) GROWTH HORMONE GENE (PhGH) IN THE ORGAN OF AFRICAN CATFISH (Clarias gariepinus) TRANSGENIC FOUNDER (Indonesian Aquaculture Journal Vol.7 No.1, 2012)

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Faster growing African catfish can be produced by transgenesis. This study was conducted to investigate the distribution and expression of growth hormone gene (PhGH) in various organs of the transgenic African catfish (Clarias gariepinus) founder (F 0 ). Transgene was detected using the PCR method in various organs, namely pituitary, brain, liver, heart, spleen, kidney, intestine, stomach, muscle, caudal fin, gill and eye. Transgene expression levels were analyzed using the method of reverse transcriptase-polymerase chain reaction (RT-PCR), -actin gene used as internal controls. The results showed that the PhGH was detected and expressed in all organs of the transgenic African catfish founder. The high level of PhGH expression was found in the liver, pituitary, intestine and brain; smaller amounts were detectable in muscle, spleen, kidneys, heart, and stomach, caudal fin, gill and eyes, range from 0.02-0.75 PhGH/-actin mRNA. The expression levels of PhGH had positive correlation with tissue and body size (P < 0.05).

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Availability

B1801316

Koleksi Digital

Archivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan Perikanan

Available

Detail Information

Series Title

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Call Number

Koleksi Digital

Publisher

2012 : KKP-Badan Penelitian dan Pengembangan Kelautan dan Perikanan., Jakarta

Collation

11-18 hlm.

Language

Indonesia

ISBN/ISSN

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Classification

NONE

Content Type

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Media Type

-

Carrier Type

-

Edition

Vol.7 No.1, 2012

Subject(s)

Jurnal
Local Content
Publikasi Internal
Clarias gariepinus
Transgenik

Specific Detail Info

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Statement of Responsibility

Indonesian Aquaculture Journal

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