Archivelago Indonesia Marine Library

Perpustakaan Kementerian Kelautan dan Perikanan Republik Indonesia

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SCREENING AND CHARACTERIZATION OF L-GLUTAMINASE PRODUCED BY BACTERIA ISOLATED FROM SANGIHE TALAUD SEA Penapisan dan Karakterisasi L-Glutaminase yang Diproduksi oleh Bakteri dari Perairan Sangihe Talaud

Usman Sumo Friend Tambunan - Personal Name; Ekowati Chasanah - Personal Name; Tanti Yulianti - Personal Name;

L-glutaminase (L-glutamine amidohydrolase, EC 3.5.1.2) is a very important enzyme due to its role as flavor enhancer and antileukemic agent. Salt-tolerant L-glutaminase produced by marine bacteria is favorable in food industries. This study describes the screening of L-glutaminase producing marine bacteria from Sangihe-Talaud Sea, North Sulawesi, Indonesia. Screening of L-glutaminase was performed using a liquid medium and identification of selected isolate was performed using molecular-based 16S rDNA. Results showed that there were 7 isolates produced positive results of L-glutaminase, and one of them (II.1 isolate) produced the highest activity, i.e 147.99 U/L, equivalent to the specific activity of 62.32 U/mg. The isolate then selected for further study. Bacterial identification based on 16S rRNA sequencing has revealed that the isolate was 96% similar to Pseudomonas aeruginosa strain CG-T8. Characterization of extracellular L-glutaminase from the II.1 isolate showed that the enzyme worked optimally at temperature of 37-45 °C and pH 7. The enzyme was stable when NaCl solution was added up to 8% and began to decrease on addition of NaCl solution of 16% and 20% with relative activity of 79% and 74%, respectively. The effect of metal ions, e.g Mn 2+ , Mg 2+ , and Co 2+ in the form of chloride salt, were able to increase enzyme activity, whereas the addition of other metal ions (Zn 2+, Fe 3+, and Ca 2+ ) decreased the activity. The molecular weights of L-glutaminase was estimated around 42 kDa and 145 kDa.


Availability
B1801648Koleksi DigitalArchivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan PerikananAvailable
Detail Information
Series Title
-
Call Number
Koleksi Digital
Publisher
2012 : KKP-Badan Penelitian dan Pengembangan Kelautan dan Perikanan., Jakarta
Collation
115-122 hlm.
Language
English
ISBN/ISSN
-
Classification
NONE
Content Type
-
Media Type
-
Carrier Type
-
Edition
Vol 7 No 3, December 2012
Subject(s)
Local Content
Publikasi Internal
Karya Ilmiah
Microbial transglutaminase
Artikel Ilmiah
Screening
Marine Bacteria
Characterization
Specific Detail Info
-
Statement of Responsibility
Squalen Journal
Other version/related

No other version available

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  • SCREENING AND CHARACTERIZATION OF L-GLUTAMINASE PRODUCED BY BACTERIA ISOLATED FROM SANGIHE TALAUD SEA Penapisan dan Karakterisasi L-Glutaminase yang Diproduksi oleh Bakteri dari Perairan Sangihe Talaud
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Archivelago Indonesia Marine Library yang berasal dari gabungan kata ‘archive’ dan ‘archipelago’ yang memiliki makna tempat yang paling sesuai dan lengkap untuk menyimpan informasi mengenai kelautan dan perikanan Indonesia, merupakan perpustakaan khusus di bawah Sekretariat Jenderal Kementerian Kelautan dan Perikanan, dengan koleksi khusus di bidang kelautan dan perikanan.

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