SUSCEPTIBILITY OF DIFFERENT CELLS TO RED SEA BREAM IRIDOVIRUS (RSIV), Indonesian Aquaculture Journal Vol.7 No.1, 2012
RSIV is an isolate virus in the genus Megalocytivirus (family Iridoviridae) that has been reported to be pathogen in more than 31 marine fish species in East Asia. The aim of study was to know the susceptibility of several cultured cells to RSIV. RSIV inoculum was inoculated onto cultured cells and then incubated in 25 o C. Routine observation of cytopatic effect (CPE) was carried out for 7 days and harvested cells were prepared for virus titration and electron microscopy (EM). The result showed that RSIV grew and propagated in GF (grunt fin), KF-1 (koi fin) and BF-2 (barfin flounder) whi ch caused cytophati c effect as cel ls enlargement. However, RSIV did not propagated on EPC (epithelioma papulosum cyprini), FHM (feathed minnow) and EK-1 (eel kidney) cells. The virus titer were 10 5.3 TCID 50 /mL in GF cells, 10 3.8 and 4.3 TCID 50 /mL in KF-1, 10 3.6 and 3.8 TCID 50 /mL in BF-2, and 7 10 2.1 TCID 50 /mL in EPC, FHM and EK-1. The EM observation revealed formation of enlarged cells containing hexagonal virus particles with 140-160 nm in diameter. These results indicated that GF was cultured cell to be optimal for replication of isolate RSIV derived from Ise bay, Mie, Japan.
|B1801382||Koleksi Digital||Archivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan Perikanan||Available|
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