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PURIFICATION AND CHARACTERIZATION OF THE NEWLY THERMOSTABLE PROTEASE PRODUCED BY Brevibacillus thermoruber LII ISOLATED FROM PADANG CERMIN HOTSPRING, INDONESIA

Thermostability is among of the vital enzyme characteristics for industrial application. Brevibacillus thermoruber LII was obtained as a potential isolate from the previous research which screened the potential thermostable protease producing bacteria from Indonesian hotspring. The newly thermostable protease produced by thermophilic Brevibacillus thermoruber LII had been purified and characterized. It was predicted that the pure enzyme obtained from Brevibacillus thermoruber LII was homo hexameric, having molecular weight of 36 kDa unit protein and its native was 215 kDa. In addition, it was also a neutral metalo serine protease according to biochemical tests that it was totaly inhibited by PMSF (Phenylmethanesulfonyl fluoride) and EDTA (Ethylenediaminetetraacetic acid). It showed optimum activity at pH of 8 and active in acidic buffer (up to pH of 4). All of metal ion in the form of chloride salt (2.5 mM) which were tested on the enzyme enhanced the enzyme activity but Li 2+. Ca 2+ ion increased the activity and the stability of enzyme against thermal. The enzyme also showed the stability against solvent. The protease LII had optimum temperature at 60 oC without CaCl 2 and 80–85 oC with addition of 2.5 mM CaCl 2. The KM and V max values for the purified protease LII were 27.2 mg/ml or 0.362 – 0.272 M for substrate HammersteinCasein (MM 75–100 kDa) and 261.1 μg/minute/ml, respectively.

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B1801821Koleksi DigitalArchivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan PerikananAvailable
Detail Information
Series Title
-
Call Number
Koleksi Digital
Publisher
2014 : KKP-Badan Penelitian dan Pengembangan Kelautan dan Perikanan.,
Collation
1-10 hlm.
Language
English
ISBN/ISSN
-
Classification
NONE
Content Type
-
Media Type
-
Carrier Type
-
Edition
9 (1), 2014
Subject(s)
Lampung
Local Content
Publikasi Internal
Karya Ilmiah
Artikel Ilmiah
Inhibitor Protease
Brevibacillus thermoruber
Specific Detail Info
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Statement of Responsibility
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