Archivelago Indonesia Marine Library

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Identification of SGS 1609 Cellulolytic Bacteria Isolated from Sargassum spec. and Characterization of The Cellulase Produced

Yusro Nuri Fawzya - Personal Name; Gintung Patantis - Personal Name; Stenny Putri - Personal Name; Nita Noriko - Personal Name;

Bacterial isolate from seaweed designated as SGS 1609 was previously found to be able to produce cellulase represented by formation of clear zone on solid medium containing carboxymethylcellulose (CMC). This research was conducted to identify the isolate and determine optimum production time as well as characterize the cellulase produced. The isolate was identified using 16s-rRNA gene analysis. Cellulase production was conducted by cultivating the isolate in the liquid medium containing CMC followed by centrifuging to get supernatant as the crude enzyme. The enzyme was then concentrated using ammonium sulfate precipitation and ultra filtration. The concentrated enzyme having higher activity produced from the concentration process was then characterized to determine its optimum pH and temperature, heat stabilization, metal ions effect
and substrate specificity. The result showed that the SGS 1609 isolate was identified as Serratia marcescens with 99% similarity. The isolate produced cellulase optimally at 4 days incubation. Ultra filtration produced higher enzyme activity compared to NH 4 -sulfate precipitation. The enzyme concentrated by ultra filtration worked optimally at the pH of 7, temperature of 50 o C, stable at the temperature of 60 o C for 240 minutes and was increased its activity by Ca 2+ and Mg 2+ ions. On the other hand, the enzyme was inhibited by Fe 3+ , Zn 2+ and Na + ions, but was not relatively affected by K + and EDTA. The use of conventional agar producer waste treated with 6% NaOH gave highest
activity compared to other substrates.


Availability
B1801795Koleksi DigitalArchivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan PerikananAvailable
Detail Information
Series Title
-
Call Number
Koleksi Digital
Publisher
2013 : KKP-Badan Penelitian dan Pengembangan Kelautan dan Perikanan., Jakarta
Collation
57-68 hlm.
Language
Indonesia
ISBN/ISSN
-
Classification
NONE
Content Type
-
Media Type
-
Carrier Type
-
Edition
8 (2), 2013
Subject(s)
Rumput Laut
Local Content
Publikasi Internal
Rumput Laut Sargassum
Bakteri Selulolitik
Enzim selulase
Specific Detail Info
-
Statement of Responsibility
Squalen Bulletin of Marine & Fisheries Postharvest & Biotechnology
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Archivelago Indonesia Marine Library yang berasal dari gabungan kata ‘archive’ dan ‘archipelago’ yang memiliki makna tempat yang paling sesuai dan lengkap untuk menyimpan informasi mengenai kelautan dan perikanan Indonesia, merupakan perpustakaan khusus di bawah Sekretariat Jenderal Kementerian Kelautan dan Perikanan, dengan koleksi khusus di bidang kelautan dan perikanan.

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