Archivelago Indonesia Marine Library

Perpustakaan Kementerian Kelautan dan Perikanan Republik Indonesia

  • Home
  • News
  • Librarian
  • Koleksi Digital
    eBook dan Teks eBook
  • Select Language :
    Arabic Bengali Brazilian Portuguese English Espanol German Indonesian Japanese Malay Persian Russian Thai Turkish Urdu

Search by :

ALL Author Subject ISBN/ISSN Advanced Search

Last search:

{{tmpObj[k].text}}
No image available for this title

PDF

CYTOTOXIC ACTIVITY AND APOPTOSIS INDUCTION OF T47D CELL LINES BY Turbinaria decurrens EXTRACT

Ekowati Chasanah - Personal Name; Nurrahmi Dewi Fajarningsih - Personal Name; Muhammad Nursid - Personal Name;

Marine algae is known to contain a wide variety of biomedical compounds having pharmaceutical applications. The aim of this research was to evaluate cytotoxic activity and apoptosis induction of Turbinaria decurrens extract on T47D cell lines. Cytotoxic activity test was conducted by using MTT assay whereas detection of apoptosis was evaluated by DNA fragmentations and flow cytometry analysis. The MTT test showed that crude extract had medium cytotoxic activity to T47D, HepG2, and C28 cell lines with IC 50 value of 172, againts 360 and 330 μg ml -1 , respectively. After solvent partition of crude extract, the cytotoxic activity of n-hexane and ethyl acetate fractions T47D cell increased, the cytotoxic activity of n. hexane and ethyl acetate fractions T47D cell increased with IC 50 value of with IC 50 43.1 and 51.9 μg ml -1 , respectively, whereas IC 50 value of methanol fraction was 383.0 μg ml -1 . Analysis of DNA fragmentation of T47D cell showed that both n-hexane and ethyl acetate fractions could not fragment DNA as a features of apoptosis. However, flow cytometry analysis by using annexin-V and propidium iodide staining revealed that n-hexane and ethyl acetate fractions could induce apoptosis in T47D cell. This research indicated that Turbinaria decurrens had potency to induce apoptosis in T47D cells.


Availability
B1801750Koleksi DigitalArchivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan PerikananAvailable
Detail Information
Series Title
-
Call Number
Koleksi Digital
Publisher
2013 : KKP-Badan Penelitian dan Pengembangan Kelautan dan Perikanan., Jakarta
Collation
23-28 hlm.
Language
Indonesia
ISBN/ISSN
-
Classification
NONE
Content Type
-
Media Type
-
Carrier Type
-
Edition
Vol 8 No 1, May 2013
Subject(s)
Sitotoksik
Local Content
Publikasi Internal
Cytotoxicity
HeLa and T47D
Specific Detail Info
-
Statement of Responsibility
Squalen Journal
Other version/related

No other version available

File Attachment
  • Please login to see this attachment
Comments

You must be logged in to post a comment

Archivelago Indonesia Marine Library
  • Information
  • Services
  • Librarian
  • Member Area
  • Katalog Integrasi
  • Pengajuan Penerbitan ISBN

About Us

Archivelago Indonesia Marine Library yang berasal dari gabungan kata ‘archive’ dan ‘archipelago’ yang memiliki makna tempat yang paling sesuai dan lengkap untuk menyimpan informasi mengenai kelautan dan perikanan Indonesia, merupakan perpustakaan khusus di bawah Sekretariat Jenderal Kementerian Kelautan dan Perikanan, dengan koleksi khusus di bidang kelautan dan perikanan.

Search

start it by typing one or more keywords for title, author or subject

Keep SLiMS Alive Want to Contribute?

© 2023 — Senayan Developer Community

Powered by SLiMS
Chat With Librarian

Please type your name before starting in conversations.


You may also hit Enter button to starting in conversation.
Select the topic you are interested in
  • Perikanan Tangkap
  • Perikanan Budidaya
  • Pengolahan Ikan
  • Pulau Kecil
  • Masyarakat Pesisir
  • Local Content
  • Ekspor Perikanan
  • Illegal Fishing
  • Ilmu - Ilmu Sosial
  • Geografi dan Sejarah
Icons made by Biro Humas dan KLN Kementerian Kelautan dan Perikanan from www.kkp.go.id
Advanced Search