CYTOTOXIC SATURATED FATTY ACIDS FROM THE INDONESIAN SEA CUCUMBER Holothuria sp
Sea cucumbers are one of Indonesia’s marine organism with the potential to be developed as possible herbal medicines. In a preliminary study of cytotoxic activity of ethanol extracts of 14 Indonesian sea cucumber species, the most active extract came from the Holothuria sp. The current research aimed to identify the major cytotoxic component in the sea cucumber Holothuria sp., that yielded the most active extract. The samples were collected from South Lampung beach. Isolation of the cytotoxic component was done employing liquid flash and preparative reversed phase (C18) chromatography. Cytotoxicity evaluation of fractions collected during the chromatography was conducted using the MCF-7 tumor cell line. Elucidation of the structure of most active isolate was done by NMR (Nuclear Magnetic Resonance) spectroscopy and GC-FID (Gas-Chromatography-Flame Ionisation Detector) analysis. The results of these analyses showed the most active compound to be stearic acid; IC 50 towards MCF-7 cells 10.32 ppm.
|B1707529||Koleksi Digital||Archivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan Perikanan||Available|
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