EFEK IMUNOSTIMULATOR ASAM LEMAK DARI BINTANG LAUT (Acanthaster Planci) TERHADAP PROLIFERASI LIMFOSIT SECARA In-Vitro, (Squalen Bulletin of Marine & Fisheries Postharvest & Biotechnology, 9 (3), 2014)
The aims of this study were to investigate lymphocyte proliferation activity and to identify chemical constituents of active fractions of starfish Acanthaster planci. Acanthaster planci was collected from Ternate Island, North Moluccas, extracted with distilled methanol and water, partitioned with gradient chloroform-hexane-methanol-water and fractionated in column chromatography using silica gel and hexane-ethyl acetate-methanol. The active compound had been purified by Thin Layer Chromatography (TLC) and identified by Gas Chromatography-Mass Spectrometry (GC-MS). The lymphocyte proliferation activity was measured based on % Stimulation Index (SI) from sample absorbency and control absorbency. The result showed that the 3 fractions of hexane fraction exhibited lymphocyte proliferation activity. Fraction 1 was able to increase lymphocyte proliferation at 48 hours and 72 hours by 55% (80 μl/ml) and 88% (160 μl/ml) increase, and fraction 2 had 77% (160 μl/ml) and 86% (640 μl/ml) increase. Meanwhile, fraction 3 had 75% (640 μl/ml) and 89% (640 μl/ml) increase. Metabolite analysis of active fraction using GC-MS yielded a number of chemical constituents that was dominated by fatty acid. The study concluded that starfish A. planci from Ternate Island has a potential source of immunostimulator.
|B1801594||Koleksi Digital||Archivelago Indonesia Marine Library - Perpustakaan Kementerian Kelautan dan Perikanan||Available|
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